Novel target screening criteria
Phylogeny
Evidence of inter-species horizontal transfer indicates potential advantage linked to resistance.
Duplication
Un-common gene copy number highlights alternate version potentially involved in resistance.
Location
Gene location in close proximity of predicted antibiotic cluster indicates potential self-resistance mechanism.
Secondary metabolite gene cluster prioritization
Known targets
Identify gene clusters that contain known antibiotic gene clusters: Automated target directed genome mining
Resistance factors
Scan gene clusters for resistance factors that have been identified using Resfams and/or custom user models
Unique metabolism
Highlight potential repurposed primary metabolism genes as well as Domains of Unknown Function (DUF) in gene cluster